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KMID : 1094720080130030269
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 3 p.269 ~ p.273
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Influence of culture modes on the microbial production of hyaluronic acid by Streptococcus zooepidemicus
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Liu Long
Du Guo-Cheng
Jian Chen
Wang Miao
Sun Jun
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Abstract
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The principal objective of this study was to assess the effects of culture modes including batch culture, pulse fed-batch culture, constant feeding rate fed-batch culture, and exponential fed-batch culture on the production of hyaluronic acid (HA) by Streptococcus zooepidemicus. Batch cultures had the highest levels of HA productivity, whereas fed-batch cultures were more favorable with regard to cell growth, and exponential fed-batch cultures evidenced the highest cell concentrations. A two-step culture model was proposed to enhance HA production: an exponential fed-batch culture was conducted prior to 8 h and then sucrose supplementation was applied for 8 h to start the batch fermentation of S. zooepidemicus. HA production and productivity were increased by 36 and 37% in the proposed two-step culture process as compared with that observed in the batch culture, respectively. The proposed two-step culture model can be applied in the production of secondary metabolites, and particularly of the exopolysaccharides.
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KEYWORD
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hyaluronic acid, fed-batch culture, culture modes, Streptococcus zooepidemicus
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